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Capacity:
Up to 10 μg of DNA.
Elution Volume:
10-50 μl.
Recover
Efficiency: Up to 95%.
Quality:
DNA purified using GenCatch
(TM) PCR Cleanup Kit is
free from enzyme, polysaccharides, agraose, carry-over slurry
and salt contamination. And is suitable for many downstream
molecular manipulations including PCR, ligation, restriction
enzyme digestion, transformation and transfection, radioactive
and fluorescent sequencing.
Fast:
Single samples can be prepared within 10 minutes with minimum efforts.
18-24 samples can be simultaneously processed in a
centrifugation format within 30 minutes. Or, more can be
processed using a vacuum manifold.
Flexible:
Mini columns can used in either centrifugation procedure or
using a vacuum manifold.
Convenient: No phenol extractions or
ethanol precipitations
required.
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GenCatch
(TM) PCR Cleanup Kit purifies DNA molecules from PCR and other
enzymatic reactions from enzymes, dNTPs, salts, and primers
without phenol/chloroform extraction. Recovery efficiency of DNA
fragments ranging from 70 bp to 10 kb in size is up to 95%. It totally
eliminate slurry carryover problem as encountered using silica
beads or glass milk based methods. Up to 10 μg
DNA can be recovered with a single column and elution volumes
can be as small as 10 μl.
PCR
products of various MW (lane 1-5) were
purified using GenCatch
(TM) PCR Cleanup Kit
and loaded as a cocktail (lane 6) onto 1.5% agraose gel. Recovery
efficiency can
be roughly estimated by comparing the overall intensity of the
individual bands.
