GenCatch
(TM) Plant RNA Purification System provides a simple, fast and cost-effective
method to purify RNA Plant and fugal samples. It
comes with shearing tubes for simple and fast homogenization and
filtration of plant tissues. It uses a simple silica-membrane
spin-column method to isolate total RNA greater than 200
nucleotides while excluding smaller RNA such as 5S, 5.8S and
tRNA without phenol/chloroform
extraction and ethanol precipitation. The system also provides
additional buffer for handling plant tissue that contai sticky
secondary metabolites. RNA isolated can be used for a variety of
molecular manipulations such as: Northern blotting, RT-PCR,
PolyA+ RNA selection, cDNA synthesis, Primer
extension and in vitro translation, etc.
Detailed
Components
High quality spin columns, collection tubes together with
buffers and protocols.
Specifications
Miniprep
Maxiprep
Sample
Size
up
tp 100 mg plant tissue
up
to 1 g of plant tissue
Binding
Capacity
up
tp 100 ug
up
to 1 mg
Elution
Volume
50
ul
500
ul
Protocol
Technical
Data
Formaldehyde
agarose gel analysis of total RNA isolated from various plant
samples with GenCatch
(TM) Plant RNA Purification System. Lane 1: Tobacco; 2:
Arabidopsis; 3: Rice; 4: Tomato leaves; 5: Tomato stem; 6:
Tomato root. 2 ug of total RNA was loaded per lane.
RT-PCR
if total RNA extracted from different tissue of tomato with GenCatch
(TM) Plant RNA Purification System. Lane 1: total RNA from
leaves; 2: total RNA from stem; 3: total RNA from root; 1 kb DNA
marker is on the left.
Formaldehyde
agarose gel analysis of total RNA isolated from various plant
samples with GenCatch
(TM) Plant RNA Purification System. Lane 1: Tobacco; 2:
Arabidopsis; 3: Rice; 4: Tomato leaves; 5: Tomato stem; 6:
Tomato root. 2 ug of total RNA was loaded per lane.
RT-PCR
if total RNA extracted from different tissue of tomato with GenCatch
(TM) Plant RNA Purification System. Lane 1: total RNA from
leaves; 2: total RNA from stem; 3: total RNA from root; 1 kb DNA
marker is on the left.
