| GenCatch
(TM) Viral RNA Mini-Prep Kit |
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| Description
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GenCatch
(TM) Viral RNA Mini-Prep Kit provides a simple, fast and cost-effective
method to purify viral nucleic acids from cell-free samples such
as plasma, serum, body fluid, urine, and cell culture
supernatant. This system utilizes guanidine thiocyanate and
2-mercaptoethanol for their capabilities to inactivate RNase and
denature proteins. Purified viral RNA is suitable fro
applications such as RT-PCR, Northern and RNase protection
assays. This system is ideal for isolation of RNA from
pathogenic viruses such as hepatitis C virus (HCV) for RT-PCR
screening of clinical samples.
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| Detailed
Components |
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High quality spin columns, collection tubes together with
buffers and protocols.
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| Downstream
Applications |
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RT-PCR. Northern
blotting. RNase protection
assay.
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| Protocol |
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| Technical
Data |
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 RT-PCR
of viral RNA isolated from infected cells with GenCatch
(TM) Viral RNA Miniprep Kit. Lane 1: mock infection; 2: 6 hr
after infection; 3: 12 hr after infection.
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